Nintendogs + Cats is a real-time pet simulator featuring several popular breeds of puppies and kittens. The game allows the player to interact with a virtual pet by speaking commands into the microphone, and by simulating physical actions with the touch screen. The animals also respond to a familiar face by use of the system's camera, allowing your pets to respond to your presence and return. Some tasks will include fulfilling everyday pet owner responsibilities like feeding your pets, walking your pets, and showing them affection. There are also several toys that can be purchased for your pet that allow for all types of games like frisbee, tug of war, and fetch the ball. There are several commands and tricks to teach your pet, which if successfully done can lead to a trophy in one of the dog competitions accessible later in the game. Other new features found in this game over the previous Nintendo DS incarnation include the ability to take 3D pictures of your pets, and much improved network and communication features that allow you meet other virtual pets in the neighborhood.

The Golden Retriever (Shiba in Japan) version of the game contains the following dog breeds:

• Golden Retriever
• Shiba
• Beagle
• Miniature Pinscher
• Dacshund
• Maltese
• Pug
• Great Dane
• Cocker Spaniel

Additionally, the game includes three types of cats; Siamese, standard and long-haired cat. The cats interact less to the player's commands, and do their own thing in comparison to the dogs' more obedient behavior.